HIV-1 env subtypes and disease progression in a cohort of HIV-1 positive individuals from Rio de Janeiro, Brazil
M.L. Guimarães1, T.C.N.F. Leite1, S.L.M. Teixeira1, V.G. Veloso2, D.P. Campos2, M.G. Morgado1
1Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil, 2Clinical Research Institute Evandro Chagas (IPEC), Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
Background: Rio de Janeiro city has a co-circulation of HIV-1 subtype B, F, variant B” of the B subtype and BF1 recombinants. Treatment and laboratory monitoring are offered for free throughout the public health system. The influence of these subtypes and HIV variants on disease progression is still a matter of controversy. Factors related to both virus and host characteristics, as well as the epidemiological context, may be of importance. Thus, the aim of this study is to define the env subtype in a cohort of HIV-1 positive patients presenting different profiles of disease progression.
Methods: The HIV-1 patients from IPEC were classified according to their progression to Aids in typical progressors (CD4>350 or clinical symptoms 5-7 after infection) and rapid progressors (CD4< 350 after < 3 years of infection). HIV-1 proviral DNA was extracted and the samples were amplified by env-gp120 nested PCR and sequenced. Sequences were manually edited and aligned with HIV-1 reference sequences by Clustal X. For phylogenetic inferences the Mega 4.0.2 program was used. The Fisher exact test was performed to verified possible correlations between HIV-1 subtypes and Aids progression.
Results: Up to now, 22 HIV-1 individuals were categorized as rapid progressors. Of them, 50% were classified as subtype B, 27,2% as B” and 22,8% as F1. From the 24 HIV-1 individuals having a typical progression to Aids subtype analyses defined 75% as B, 20,8% as B” and 4,2% as C. Preliminary data point to a statistically significant association of rapid progression to Aids and HIV-1 subtype F1 infection (p = 0,0192).
Conclusions: If the HV-1 subtype F1 is associated to a shorter individual survival and, as a result, these strains would be less transmitted in the population, this could explain the low rate (10-15%) maintained for this subtype in Rio de Janeiro.
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