XVIII International AIDS Conference

Abstract

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HTLV-1 modulates the in vitro cytokines production in HIV-1 coinfected patients

M. Abrahão, R. Lima, E. Netto, C. Brites

Federal University of Bahia, Virology Research Laboratory, Salvador, Brazil

Background: The impact of HTLV-1 coinfection on natural history of HIV-1 infection is controversial. There is scarce data on the impact of coinfection on patient´s immune response.
Methods: Eighty individuals were included in the study: 33 infected by HIV-1, 15 infected by HTLV-1, 19 coinfected by both agents, and 13 healthy volunteers. Two million PBMC were separated from whole blood by gradient of centrifugation, and cultured in RPMI for 72 h. Supernatant aliquots were harvested at 24, 48 and 72 h, and tested for IL-2, IL-4, IL-6, IL-10 and gamma-IFN. CD4+ cells count did not differ for all groups. All patients were asymptomatic during the study period.
Results: We found a significant difference in the production of IL-2, IL-10 and γ -IFN at all time points, and also in IL-4 production at 48 h, as showed in the table below (median values, pg/ml).


GroupIL-2*IL-4**IL-6*IL-10**γ-IFN*
HIV5.423.325.5>2.0005.5
HTLV-114.38.815.3>2.00010.2
Coinfected17.920.323.4>2.00011.0
Controls-7.822.236.440.45.7
[Table 1]


* p< 0.01
Coinfected patients showed a higher production of Il-2, and γ-IFN. In addition, they also produced more IL-4 and IL-6 than HTLV-1 infected patients. All infected patients had higher IL-10 production, compared with controls. High levels (above median) of IL-4 were detected in all groups, but HTLV-1 group showed a greater proportion of patients with high levels of IL-10, IL-2 and γ-IFN. Patients infected by HIV-1 had a larger proportion of higher producers of IL-4 and IL-6, while coinfected patients had a pattern of high levels of all cytokynes, but IL-6.
Conclusions: HTLV-1 coinfection modulates the cytokynes production in HIV-1-infected individuals, with a predominant Th1 response. These findings may help to understand the role of coinfection on AIDS progression.


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